User profiles for JD Bloom
Jesse D. BloomFred Hutch Cancer Center; University of Washington; Howard Hughes Medical Institute Verified email at fredhutch.org Cited by 23209 |
In the light of directed evolution: pathways of adaptive protein evolution
Directed evolution is a widely-used engineering strategy for improving the stabilities or
biochemical functions of proteins by repeated rounds of mutation and selection. These …
biochemical functions of proteins by repeated rounds of mutation and selection. These …
[PDF][PDF] Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding
The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein mediates viral
attachment to ACE2 receptor and is a major determinant of host range and a dominant target …
attachment to ACE2 receptor and is a major determinant of host range and a dominant target …
[PDF][PDF] Complete mapping of mutations to the SARS-CoV-2 spike receptor-binding domain that escape antibody recognition
Antibodies targeting the SARS-CoV-2 spike receptor-binding domain (RBD) are being developed
as therapeutics and are a major contributor to neutralizing antibody responses elicited …
as therapeutics and are a major contributor to neutralizing antibody responses elicited …
Within-host evolution of human influenza virus
The rapid global evolution of influenza virus begins with mutations that arise de novo in
individual infections, but little is known about how evolution occurs within hosts. We review …
individual infections, but little is known about how evolution occurs within hosts. We review …
[PDF][PDF] Safety and efficacy of bilateral submental cryolipolysis with quantified 3-dimensional imaging of fat reduction and skin tightening
EF Bernstein, JD Bloom - JAMA facial plastic surgery, 2017 - jamanetwork.com
Importance Cryolipolysis is a popular, well-tolerated nonsurgical procedure that uses
controlled cooling to selectively destroy fat cells. Central submental cryolipolysis has been …
controlled cooling to selectively destroy fat cells. Central submental cryolipolysis has been …
[HTML][HTML] Defining the risk of SARS-CoV-2 variants on immune protection
The global emergence of many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
variants jeopardizes the protective antiviral immunity induced after infection or …
variants jeopardizes the protective antiviral immunity induced after infection or …
Evolving strategies for enzyme engineering
Directed evolution is a common technique to engineer enzymes for a diverse set of applications.
Structural information and an understanding of how proteins respond to mutation and …
Structural information and an understanding of how proteins respond to mutation and …
[HTML][HTML] SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape
An ideal therapeutic anti-SARS-CoV-2 antibody would resist viral escape 1 , 2 – 3 , have
activity against diverse sarbecoviruses 4 , 5 , 6 – 7 , and be highly protective through viral …
activity against diverse sarbecoviruses 4 , 5 , 6 – 7 , and be highly protective through viral …
Protein stability promotes evolvability
JD Bloom, ST Labthavikul, CR Otey… - Proceedings of the …, 2006 - National Acad Sciences
The biophysical properties that enable proteins to so readily evolve to perform diverse
biochemical tasks are largely unknown. Here, we show that a protein’s capacity to evolve is …
biochemical tasks are largely unknown. Here, we show that a protein’s capacity to evolve is …
[PDF][PDF] Comprehensive mapping of mutations in the SARS-CoV-2 receptor-binding domain that affect recognition by polyclonal human plasma antibodies
The evolution of SARS-CoV-2 could impair recognition of the virus by human antibody-mediated
immunity. To facilitate prospective surveillance for such evolution, we map how …
immunity. To facilitate prospective surveillance for such evolution, we map how …