User profiles for JD Bloom

Jesse D. Bloom

Fred Hutch Cancer Center; University of Washington; Howard Hughes Medical Institute
Verified email at fredhutch.org
Cited by 23209

In the light of directed evolution: pathways of adaptive protein evolution

JD Bloom, FH Arnold - … of the National Academy of Sciences, 2009 - National Acad Sciences
Directed evolution is a widely-used engineering strategy for improving the stabilities or
biochemical functions of proteins by repeated rounds of mutation and selection. These …

[PDF][PDF] Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding

…, MA Tortorici, AC Walls, NP King, D Veesler, JD Bloom - cell, 2020 - cell.com
The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein mediates viral
attachment to ACE2 receptor and is a major determinant of host range and a dominant target …

[PDF][PDF] Complete mapping of mutations to the SARS-CoV-2 spike receptor-binding domain that escape antibody recognition

…, SPJ Whelan, RH Carnahan, JE Crowe, JD Bloom - Cell host & …, 2021 - cell.com
Antibodies targeting the SARS-CoV-2 spike receptor-binding domain (RBD) are being developed
as therapeutics and are a major contributor to neutralizing antibody responses elicited …

Within-host evolution of human influenza virus

KS Xue, LH Moncla, T Bedford, JD Bloom - Trends in Microbiology, 2018 - cell.com
The rapid global evolution of influenza virus begins with mutations that arise de novo in
individual infections, but little is known about how evolution occurs within hosts. We review …

[PDF][PDF] Safety and efficacy of bilateral submental cryolipolysis with quantified 3-dimensional imaging of fat reduction and skin tightening

EF Bernstein, JD Bloom - JAMA facial plastic surgery, 2017 - jamanetwork.com
Importance Cryolipolysis is a popular, well-tolerated nonsurgical procedure that uses
controlled cooling to selectively destroy fat cells. Central submental cryolipolysis has been …

[HTML][HTML] Defining the risk of SARS-CoV-2 variants on immune protection

…, G Alter, RR Amara, RS Baric, DH Barouch, JD Bloom… - Nature, 2022 - nature.com
The global emergence of many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
variants jeopardizes the protective antiviral immunity induced after infection or …

Evolving strategies for enzyme engineering

JD Bloom, MM Meyer, P Meinhold, CR Otey… - Current opinion in …, 2005 - Elsevier
Directed evolution is a common technique to engineer enzymes for a diverse set of applications.
Structural information and an understanding of how proteins respond to mutation and …

[HTML][HTML] SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape

…, HW Virgin, SPJ Whelan, D Veesler, D Corti, JD Bloom… - Nature, 2021 - nature.com
An ideal therapeutic anti-SARS-CoV-2 antibody would resist viral escape 1 , 2 – 3 , have
activity against diverse sarbecoviruses 4 , 5 , 6 – 7 , and be highly protective through viral …

Protein stability promotes evolvability

JD Bloom, ST Labthavikul, CR Otey… - Proceedings of the …, 2006 - National Acad Sciences
The biophysical properties that enable proteins to so readily evolve to perform diverse
biochemical tasks are largely unknown. Here, we show that a protein’s capacity to evolve is …

[PDF][PDF] Comprehensive mapping of mutations in the SARS-CoV-2 receptor-binding domain that affect recognition by polyclonal human plasma antibodies

…, TN Starr, KD Malone, HY Chu, JD Bloom - Cell host & …, 2021 - cell.com
The evolution of SARS-CoV-2 could impair recognition of the virus by human antibody-mediated
immunity. To facilitate prospective surveillance for such evolution, we map how …